2CFX

Structure of B.subtilis LrpC

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	C 2 2 21
格子定数 [Å]	165.834, 166.151, 155.768
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	119.520 - 2.400
R因子	0.217
Rwork	0.215
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	LRPA
結合長の平均二乗偏差(RMSD) [Å]	0.027
結合角の平均二乗偏差(RMSD) [度]	2.325
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	30.000	2.530
分解能 [Å] (高)	2.400	2.400
Rmerge_l_obs	0.090	0.490
独立反射数	83371
<I/σ(I)>	9.6	1.9
完全性 [%]	99.4	99.8

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1		8.6		pH 8.60