2AXJ
Crystal structures of T cell receptor beta chains related to rheumatoid arthritis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-10-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 187.478, 187.478, 86.679 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.870 - 2.650 |
R-factor | 0.233 |
Rwork | 0.233 |
R-free | 0.28600 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2axh |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.740 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.075 | 0.614 |
Number of reflections | 25627 | |
<I/σ(I)> | 17.8 | 1.8 |
Completeness [%] | 96.1 | 77.5 |
Redundancy | 3.9 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.6-0.7 M sodium citrate, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |