2AXH
Crystal structures of T cell receptor beta chains related to rheumatoid arthritis
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 187.334, 187.334, 86.279 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.180 - 2.700 |
R-factor | 0.237 |
Rwork | 0.237 |
R-free | 0.28000 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.200 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.075 | 0.478 |
Number of reflections | 24387 | |
<I/σ(I)> | 35 | 5.2 |
Completeness [%] | 97.6 | 99.6 |
Redundancy | 6.6 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.6-0.7m sodium citrate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.6-0.7m sodium citrate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.6-0.7m sodium citrate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |