2AXH

Crystal structures of T cell receptor beta chains related to rheumatoid arthritis

Experimental procedure

実験手法	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-08
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9794
Spacegroup name	P 63 2 2
格子定数 [Å]	187.334, 187.334, 86.279
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	39.180 - 2.700
R因子	0.237
Rwork	0.237
R-free	0.28000
Structure solution method	MAD
結合長の平均二乗偏差(RMSD) [Å]	0.007
結合角の平均二乗偏差(RMSD) [度]	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	39.200	2.800
分解能 [Å] (高)	2.700	2.700
Rmerge_l_obs	0.075	0.478
独立反射数	24387
<I/σ(I)>	35	5.2
完全性 [%]	97.6	99.6
冗長性	6.6	6.3

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.6-0.7m sodium citrate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.6-0.7m sodium citrate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.6-0.7m sodium citrate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K