208D
HIGH-RESOLUTION STRUCTURE OF A DNA HELIX FORMING (C.G)*G BASE TRIPLETS
Experimental procedure
Source type | ROTATING ANODE |
Temperature [K] | 291 |
Detector technology | IMAGE PLATE |
Collection date | 1994-05-02 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
格子定数 [Å] | 22.238, 37.010, 54.100 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 8.000 - 2.050 |
R因子 | 0.168 |
Rwork | 0.168 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | BDL001 |
結合長の平均二乗偏差(RMSD) [Å] | 0.015 |
結合角の平均二乗偏差(RMSD) [度] | 25.500 * |
Data reduction software | IPMOSFLM |
Phasing software | AMoRE |
Refinement software | X-PLOR |
Quality characteristics
Overall | |
分解能 [Å] (高) | 2.050 * |
Rmerge_l_obs | 0.036 |
独立反射数 | 2824 |
完全性 [%] | 87.0 |
冗長性 | 7.9 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION | 6 | 16 * | pH 6.00, VAPOR DIFFUSION, temperature 289.00K |
結晶化試薬
ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | SPERMINE | ||
5 | 1 | 2 | WATER | ||
6 | 1 | 2 | MPD |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | drop | DNA | 0.5 (mM) | |
2 | 1 | drop | 54 (mM) | ||
3 | 1 | drop | spermine | 2.4 (mM) | |
4 | 1 | drop | MPD | 11 (%) | |
5 | 1 | reservoir | MPD | 50 (%) |