1ZZS
Bovine eNOS N368D single mutant with L-N(omega)-Nitroarginine-(4R)-Amino-L-Proline Amide Bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.870, 107.060, 156.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.560 - 1.850 |
R-factor | 0.175 |
Rwork | 0.175 |
R-free | 0.21100 |
Structure solution method | Difference Fourier |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 82165 | |
<I/σ(I)> | 11.7 | 2 |
Completeness [%] | 97.8 | 99.2 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 280 | PEG3350, sodium cacodylate, magnesium acetate, TCEP, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 280K |