1ZVK
Structure of Double mutant, D164N, E78H of Kumamolisin-As
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-07-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.050, 74.810, 78.380 |
Unit cell angles | 90.00, 103.08, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.040 |
R-factor | 0.1885 |
Rwork | 0.188 |
R-free | 0.29180 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.029 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 2.030 | 2.030 |
Number of reflections | 41268 | |
<I/σ(I)> | 14.9 | 4.7 |
Completeness [%] | 97.9 | 79.2 |
Redundancy | 3.5 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | PEG8000, Ammounium Sulfate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |