1ZNN
Structure of the synthase subunit of PLP synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-17 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97951 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 162.531, 163.917, 186.804 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.940 - 2.200 |
R-factor | 0.16117 |
Rwork | 0.159 |
R-free | 0.19804 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.564 |
Data reduction software | HKL-2000 |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHELXS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.200 |
Number of reflections | 125880 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | PEG 8000, (NH4)2SO4, cacodylate, pH 7.0, VAPOR DIFFUSION, temperature 293K |