1Z14
Structural Determinants of Tissue Tropism and In Vivo Pathogenicity for the Parvovirus Minute Virus of Mice
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Wavelength(s) | 0.870 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 448.700, 416.500, 306.100 |
Unit cell angles | 90.00, 95.90, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.250 |
Rwork | 0.298 |
R-free | 0.30600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | canine parvovirus model |
RMSD bond length | 0.010 |
RMSD bond angle | 1.583 |
Data scaling software | SCALEPACK |
Phasing software | GLRF |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 3.250 |
Rmerge | 0.162 |
Number of reflections | 517599 |
Completeness [%] | 64.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 10mM Tris-HCL, 8 mM CaCl2, PEG8000 and virus 10 mgs/ml, pH 7.5, VAPOR DIFFUSION, HANGING DROP | |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 10mM Tris-HCL, 8 mM CaCl2, PEG8000 and virus 10 mgs/ml, pH 7.5, VAPOR DIFFUSION, HANGING DROP |