1YHW
Crystal Structure of PAK1 kinase domain with one point mutations (K299R)
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2000-01-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| 格子定数 [Å] | 51.763, 103.049, 122.639 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 50.000 - 1.800 |
| R因子 | 0.2262 |
| Rwork | 0.226 |
| R-free | 0.24350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 50.000 |
| 分解能 [Å] (高) | 1.800 |
| Rmerge_l_obs | 0.073 |
| 独立反射数 | 28485 |
| <I/σ(I)> | 16.9 |
| 完全性 [%] | 92.4 |
| 冗長性 | 4.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG 4000, NaCl, PIPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
