1Y3T
Crystal structure of YxaG, a dioxygenase from Bacillus subtilis
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| 格子定数 [Å] | 128.417, 128.425, 51.189 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 91.290 - 2.400 |
| R因子 | 0.22013 |
| Rwork | 0.217 |
| R-free | 0.27679 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gqg |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.047 |
| 結合角の平均二乗偏差(RMSD) [度] | 2.996 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 20.000 | 2.500 |
| 分解能 [Å] (高) | 2.400 | 2.400 |
| 独立反射数 | 33292 | |
| <I/σ(I)> | 20.37 | |
| 完全性 [%] | 98.2 | 94.3 |
| 冗長性 | 6.01 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Sodium Hepes, PEG 400, Ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
