1XJV
Crystal structure of human POT1 bound to telomeric single-stranded DNA (TTAGGGTTAG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 160 |
Detector technology | CCD |
Collection date | 2003-12-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.107 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 102.026, 103.243, 71.664 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.290 - 1.730 |
R-factor | 0.232 |
Rwork | 0.228 |
R-free | 0.23700 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.390 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.290 | 1.790 |
High resolution limit [Å] | 1.730 | 1.730 |
Number of reflections | 38238 | |
<I/σ(I)> | 15.2 | |
Completeness [%] | 95.4 | 77.2 |
Redundancy | 7.1 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289 | Ammonium sulfate, DTT, Sodium Chloride, Trisodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289 | Ammonium sulfate, DTT, Sodium Chloride, Trisodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289 | Ammonium sulfate, DTT, Sodium Chloride, Trisodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | (NH4)2SO4 | ||
2 | 1 | 1 | NaCl | ||
3 | 1 | 1 | sodium citrate | ||
4 | 1 | 1 | DTT | ||
5 | 1 | 2 | (NH4)2SO4 | ||
6 | 1 | 2 | NaCl | ||
7 | 1 | 2 | sodium citrate | ||
8 | 1 | 2 | DTT |