1WAJ
DNA POLYMERASE FROM BACTERIOPHAGE RB69
Experimental procedure
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-09 |
| Detector | MACSCIENCE |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 81.410, 117.600, 199.240 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 40.000 - 2.800 |
| R因子 | 0.21 |
| Rwork | 0.210 |
| R-free | 0.28000 |
| Structure solution method | MIR |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.015 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 40.000 |
| 分解能 [Å] (高) | 2.800 |
| Rmerge_l_obs | 0.089 |
| 独立反射数 | 42608 |
| 完全性 [%] | 89.0 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, sitting drop * | 5.6 | 4 * | pH 5.6 |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | drop | sodium citrate | 0.05 (M) | |
| 3 | 1 | drop | sodium potassium tartrate | 0.025 (M) | |
| 4 | 1 | drop | ammonium sulfate | 0.5 (M) | |
| 5 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 6 | 1 | reservoir | sodium potassium tartrate | 0.5 (M) | |
| 7 | 1 | reservoir | ammonium sulfate | 1.0 (M) |
