1W98
The structural basis of CDK2 activation by cyclin E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-01 |
Detector | ADSC CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 99.620, 99.620, 149.987 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 83.050 - 2.150 |
R-factor | 0.185 |
Rwork | 0.181 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jst |
RMSD bond length | 0.018 |
RMSD bond angle | 1.690 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.280 | 2.210 |
High resolution limit [Å] | 2.130 | 2.130 |
Rmerge | 0.080 | 0.480 |
Number of reflections | 39329 | |
<I/σ(I)> | 12.8 | 1.9 |
Completeness [%] | 94.2 | 53.8 |
Redundancy | 4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | PCDK2/CYCLIN E (6-8 MG/ML), 1 MM AMPPNP, 10-15% PEG3350, 0.2 M SODIUM CITRATE PH7.5, pH 7.50 |