1VWB

STREPTAVIDIN-CYCLO-AC-[CHPQFC]-NH2, PH 11.8

Experimental procedure

Temperature [K]	293
Detector technology	AREA DETECTOR
Detector	SIEMENS
Spacegroup name	I 41 2 2
Unit cell lengths	59.250, 59.250, 178.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 1.820
R-factor	0.183
Rwork	0.183
R-free	0.23900
RMSD bond length	0.017
RMSD bond angle	27.600 *
Data reduction software	SADIE
Data scaling software	SAINT
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.720 *
Rmerge	0.075
Total number of observations	49797 *
Number of reflections	13754
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	11.8	20 *	Pahler, A., (1987) J. Biol. Chem., 262, 13933. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	drop	potassium acetate	50 (mM)
3	1	drop		200 (mM)	pH4.5
4	1	reservoir	ammonium sulfate	30 (%sat)
5	1	reservoir	potassium acetate	50 (mM)
6	1	reservoir		200 (mM)