1UW7
Nsp9 protein from SARS-coronavirus.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8856, 0.97829, 0.99988 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 58.000, 58.000, 85.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
R-factor | 0.228 |
Rwork | 0.228 |
R-free | 0.29400 * |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.700 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE/RESOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.800 | 3.300 * |
Rmerge | 0.190 | 0.480 * |
Number of reflections | 3908 | |
<I/σ(I)> | 20 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 24. * | 25. * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 100MM CITRATE/PHOSPHATE PH3.8, 20% PEG 8000, PROTEIN CONCENTRATION 10MG/ML SITTING DROP 100NL + 100NL, pH 3.80 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 10 (mM) | |
2 | 1 | drop | 100 (mM) | ||
3 | 1 | drop | dithiothreitol | 2 (mM) | pH8.0 |
4 | 1 | drop | protein | 10 (mg/ml) | |
5 | 1 | reservoir | citrate phosphate | 100 (mM) | pH3.8 |
6 | 1 | reservoir | PEG8000 | 20 (%) |