1UVC
Lipid Binding in Rice Nonspecific Lipid Transfer Protein-1 Complexes from Oryza sativa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 160 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-07-15 |
| Detector | MSC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.940, 53.800, 49.080 |
| Unit cell angles | 90.00, 96.36, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mzm |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.180 |
| Number of reflections | 12898 | |
| <I/σ(I)> | 18.3 | 7.5 |
| Completeness [%] | 96.2 | 92.4 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | POLYETHYLENE GLYCOL 600, pH 5.60 |
