1UHI
Crystal structure of i-aequorin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | OXFORD PX210 |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 |
Unit cell lengths | 34.263, 54.432, 57.523 |
Unit cell angles | 102.05, 99.70, 103.80 |
Refinement procedure
Resolution | 33.270 - 1.800 |
R-factor | 0.19126 |
Rwork | 0.188 |
R-free | 0.25035 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ej3 |
RMSD bond length | 0.021 |
RMSD bond angle | 1.900 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.270 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 35601 | |
<I/σ(I)> | 8.3 | |
Completeness [%] | 96.7 | 96.7 |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | ammonium sulfate, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |