1U1D
Structure of e. coli uridine phosphorylase complexed to 5-(phenylthio)acyclouridine (ptau)
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 8-BM |
Synchrotron site | APS |
Beamline | 8-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9790 |
Spacegroup name | P 21 21 21 |
格子定数 [Å] | 91.150, 125.746, 140.880 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 47.140 - 2.001 |
R因子 | 0.21098 |
Rwork | 0.209 |
R-free | 0.22635 |
Structure solution method | MOLECULAR REPLACEMENT |
結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
結合角の平均二乗偏差(RMSD) [度] | 0.891 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.1.24) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 47.140 | 2.070 |
分解能 [Å] (高) | 2.000 | 2.000 |
独立反射数 | 109591 | |
完全性 [%] | 99.9 | 99.9 |
冗長性 | 7.3 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 295 | PEG 4000, MES, GLYCEROL, PH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |