1SFS
1.07 A crystal structure of an uncharacterized B. stearothermophilus protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-27 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.319, 70.090, 74.157 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.070 |
Rwork | 0.102 |
R-free | 0.13520 |
Structure solution method | SIRAS |
RMSD bond length | 0.014 |
RMSD bond angle | 0.031 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.110 |
High resolution limit [Å] | 1.070 | 1.070 |
Rmerge | 0.059 | 0.287 |
Number of reflections | 90332 | |
<I/σ(I)> | 20.24 | 6.64 |
Completeness [%] | 94.5 | 73.7 |
Redundancy | 8.53 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.2M Ammonium Phosphate, 20% PEG, 0.1M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |