1RS9
Bovine endothelial NOS heme domain with D-phenylalanine-D-nitroarginine amide bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-10-23 |
Detector | MARRESEARCH |
Wavelength(s) | 1.08 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.791, 106.647, 157.659 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.500 - 2.220 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.26400 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.300 |
High resolution limit [Å] | 2.220 | 2.220 |
Rmerge | 0.069 | 0.450 |
Number of reflections | 49047 | |
<I/σ(I)> | 31.4 | 3.4 |
Completeness [%] | 99.1 | 94 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 280 | PEG 3350, MgOAc, Na Cacodylate, TCEP, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 280K |