1RKA

THE APO FORM OF E. COLI RIBOKINASE

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-05-15
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
格子定数 [Å]	61.970, 61.970, 127.834
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	15.000 - 2.300
Rwork	0.210
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1RKD: RESIDUES 4-9 44-93 123-241 AND 254-309.
結合長の平均二乗偏差(RMSD) [Å]	0.006 *
結合角の平均二乗偏差(RMSD) [度]	1.450 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.3)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	15.000	2.340
分解能 [Å] (高)	2.300	2.300
Rmerge_l_obs	0.056	0.235
Total number of observations	62741 *
独立反射数	12644
<I/σ(I)>	20.6	6.7
完全性 [%]	96.4	87.5
冗長性	5.5	5.5

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, hanging drop *	4.8		drop consists of equal volume of protein and reservoir solutions *

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	ribokinase	11 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop		0.2 (M)
4	1	reservoir	Na acetate	0.1 (M)
5	1	reservoir	NH4 acetate	0.2 (M)
6	1	reservoir	mPEG2000	22-33 (%)