1RKA
THE APO FORM OF E. COLI RIBOKINASE
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-05-15 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
格子定数 [Å] | 61.970, 61.970, 127.834 |
格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
残基 | 15.000 - 2.300 |
Rwork | 0.210 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1RKD: RESIDUES 4-9 44-93 123-241 AND 254-309. |
結合長の平均二乗偏差(RMSD) [Å] | 0.006 * |
結合角の平均二乗偏差(RMSD) [度] | 1.450 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.3) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 15.000 | 2.340 |
分解能 [Å] (高) | 2.300 | 2.300 |
Rmerge_l_obs | 0.056 | 0.235 |
Total number of observations | 62741 * | |
独立反射数 | 12644 | |
<I/σ(I)> | 20.6 | 6.7 |
完全性 [%] | 96.4 | 87.5 |
冗長性 | 5.5 | 5.5 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | Vapor diffusion, hanging drop * | 4.8 | drop consists of equal volume of protein and reservoir solutions * |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | drop | ribokinase | 11 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | drop | 0.2 (M) | ||
4 | 1 | reservoir | Na acetate | 0.1 (M) | |
5 | 1 | reservoir | NH4 acetate | 0.2 (M) | |
6 | 1 | reservoir | mPEG2000 | 22-33 (%) |