1REO
L-amino acid oxidase from Agkistrodon halys pallas
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 300 |
Wavelength(s) | 0.8883 |
Spacegroup name | I 21 3 |
格子定数 [Å] | 169.310, 169.310, 169.310 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 50.000 * - 2.310 |
R因子 | 0.194 |
Rwork | 0.194 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f8r |
結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
結合角の平均二乗偏差(RMSD) [度] | 22.400 * |
Data reduction software | AUTOMAR |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 50.000 | |
分解能 [Å] (高) | 2.310 * | 2.310 * |
Rmerge_l_obs | 0.054 * | 0.383 * |
Total number of observations | 247448 * | |
独立反射数 | 34867 | |
完全性 [%] | 98.2 * | 99.8 * |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 300 | ammonium sulfate, citrate soldium, nikel sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | drop | protein | 44 (mg/ml) | in double-distilled water |
2 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
3 | 1 | reservoir | ammonium sulfate | 2 (M) | |
4 | 1 | reservoir | 10 (mM) |