1QJA
14-3-3 ZETA/PHOSPHOPEPTIDE COMPLEX (MODE 2)
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 68.380, 70.956, 130.560 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 15.000 - 2.000 |
| R因子 | 0.214 * |
| Rwork | 0.214 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 14-3-3 ZETA |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.033 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 2.090 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.064 | 0.269 |
| 独立反射数 | 41523 | |
| <I/σ(I)> | 18.5 | 3.9 |
| 完全性 [%] | 95.2 | 94.4 |
| 冗長性 | 4.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 6.2 | 4 * | used to seeding * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | reservoir | PIPES | 100 (mM) | |
| 2 | 1 | reservoir | 20 (mM) | ||
| 3 | 1 | reservoir | isopropanol | 15-17 (%) | |
| 4 | 1 | reservoir | PEG4000 | 12 (%) |
