1QJ9

CRYSTAL STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX FROM ESCHERICHIA COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-04-15
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	82.300, 82.300, 208.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.000 - 2.100
R-factor	0.233 *
Rwork	0.233
R-free	0.28400
Structure solution method	MIR
RMSD bond length	0.021
RMSD bond angle	0.041
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.076 *	0.320 *
Number of reflections	16165
<I/σ(I)>	11	2.7
Completeness [%]	99.0	99
Redundancy	3.6	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *		30 % (V/V) 2-PROPANOL, 20 % (V/V) GLYCEROL, 0.2 M CACL2, 0.1 M NA-ACETATE PH 4.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	2-propanol	30 (%(v/v))
2	1	reservoir	glycerol	20 (%(v/v))
3	1	reservoir		0.2 (M)
4	1	reservoir	sodium acetate	0.1 (M)
5	1	drop	protain	20 (mg/ml)
6	1	drop		0.6 (%(v/v))
7	1	drop	Tris-HCl	20 (mM)