1QDD
CRYSTAL STRUCTURE OF HUMAN LITHOSTATHINE TO 1.3 A RESOLUTION
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| 格子定数 [Å] | 48.000, 48.000, 111.000 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 20.000 - 1.300 |
| R因子 | 0.132 |
| R-free | 0.15900 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 20.000 | |
| 分解能 [Å] (高) | 1.300 | 1.300 |
| Rmerge_l_obs | 0.084 | 0.280 |
| Total number of observations | 114712 * | |
| 独立反射数 | 32253 | |
| 完全性 [%] | 95.4 | 96.2 |
| 冗長性 | 2.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 20 * | Pignol, D., (1995) Proteins: Struct.,Funct., Genet., 23, 604. * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.0 |
| 2 | 1 | reservoir | PEG4000 | 12 (%(w/v)) | |
| 3 | 1 | drop | protein | 7 (mg/ml) | |
| 4 | 1 | drop | sodium acetate | 50 (mM) | |
| 5 | 1 | drop | PEG4000 | 6 (%(w/v)) |
