1P5Y
The structures of host range controlling regions of the capsids of canine and feline parvoviruses and mutants
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 440.450, 246.810, 443.650 |
| Unit cell angles | 90.00, 93.54, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.200 |
| Rwork | 0.252 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.520 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.600 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.143 * | 0.297 * |
| Number of reflections | 474357 | |
| Completeness [%] | 70.5 * | 54.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 20 * | PEG8000, CaCl2, Tris.HCL, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 20 * | PEG8000, CaCl2, Tris.HCL, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 20 * | PEG8000, CaCl2, Tris.HCL, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 20 * | PEG8000, CaCl2, Tris.HCL, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 1 (%) | |
| 2 | 1 | reservoir | 8 (mM) | ||
| 3 | 1 | reservoir | Tris-HCl | 20 (mM) | pH7.2 |
