1OGK
The crystal structure of Trypanosoma cruzi dUTPase in complex with dUDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.386, 50.307, 111.387 |
| Unit cell angles | 90.00, 101.35, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.900* |
| R-factor | 0.209 |
| Rwork | 0.205 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NATIVE STRUCTURE |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.930 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.13) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.085 | 0.442 |
| Number of reflections | 27211 | |
| <I/σ(I)> | 9.46 | 1.1 |
| Completeness [%] | 99.7 | 97.3 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 * | 17 * | 25% PEG 2000 MME, 0.3M SODIUM FORMATE, 50 MM SODIUM HEPES PH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium formate | 0.3 (M) | |
| 2 | 1 | reservoir | PEG2000 MME | 25 (%) | |
| 3 | 1 | reservoir | sodium HEPES | 50 (mM) | pH7.0 |
