1O63
Crystal structure of an ATP phosphoribosyltransferase
Experimental procedure
実験手法 | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Detector technology | CCD |
Detector | MARRESEARCH |
Wavelength(s) | 0.9795 , 1.5418 |
Spacegroup name | P 1 21 1 |
格子定数 [Å] | 53.257, 49.972, 76.403 |
格子定数 [度] | 90.00, 92.75, 90.00 |
精密化法
残基 | 25.910 - 2.000 |
Rwork | 0.219 |
R-free | 0.26400 |
Structure solution method | Se-Met MAD phasing |
結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
結合角の平均二乗偏差(RMSD) [度] | 1.800 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA) |
Refinement software | REFMAC (4.0) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 25.910 | 2.110 |
分解能 [Å] (高) | 2.000 | 2.000 |
Rmerge_l_obs | 0.070 | 0.178 |
独立反射数 | 27249 | |
<I/σ(I)> | 14.3 | 8.1 |
完全性 [%] | 99.5 | 99.5 |
冗長性 | 4.9 | 4.8 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | Vapor diffusion, hanging drop * | 7.5 | 293 |
結晶化試薬
ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
1 | 1 | 1 | protein | 21.6mg/ml | |
2 | 1 | 2 | Tris | 20mM | |
3 | 1 | 2 | NaCl | 150mM | |
4 | 1 | 2 | beta-mercaptoethanol | 1mM | |
5 | 1 | 2 | methionine | 10mM |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
2 | 1 | drop | 150 (mM) | ||
3 | 1 | drop | methionine | 10 (mM) | |
4 | 1 | drop | glycerol | 10 (%) | |
5 | 1 | drop | dithiothreitol | 5 (mM) | |
6 | 1 | drop | protein | 10 (mg/ml) |