1O0R

Crystal structure of the catalytic domain of bovine beta1,4-galactosyltransferase complex with UDP-galactose

Replaces:  1KYB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-10-09
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	50.356, 91.775, 142.467
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.800 *
R-factor	0.203
Rwork	0.203
R-free	0.28000 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009 *
RMSD bond angle	1.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.380
High resolution limit [Å]	2.800 *	2.300
Rmerge	0.176 *	0.650 *
Total number of observations	58965 *
Number of reflections	15985 *
<I/σ(I)>	15.3	2.4
Completeness [%]	98.5	93 *
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	Mes, dioxane, ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Gal-T1	20 (mg/ml)
2	1	drop	UDP-Gal	17 (mM)
3	1	drop		17 (mM)
4	1	reservoir	dioxane	10 (%(v/v))
5	1	reservoir	MES-NaOH	100 (mM)	pH6.5
6	1	reservoir	ammonium sulfate	2-2.5 (M)