1NLQ
The crystal structure of Drosophila NLP-core provides insight into pentamer formation and histone binding
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-04-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97950 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.608, 60.089, 73.426 |
Unit cell angles | 90.00, 90.79, 90.00 |
Refinement procedure
Resolution | 100.000 * - 1.550* |
Rwork | 0.234 |
R-free | 0.25400 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.030 * | 0.265 |
Total number of observations | 478081 * | |
Number of reflections | 80828 | |
<I/σ(I)> | 39.5 | 6.7 |
Completeness [%] | 97.3 | 94.5 |
Redundancy | 5.9 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | PEG 400, sodium chloride, magnesium chloride, tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | PEG 400, sodium chloride, magnesium chloride, tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | PEG400 | 30-33 (%) | |
3 | 1 | reservoir | 20 (mM) |