1N34
Structure of the Thermus thermophilus 30S ribosomal subunit in the presence of codon and crystallographically disordered near-cognate transfer rna anticodon stem-loop mismatched at the first codon position
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2001-05-05 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 41 21 2 |
格子定数 [Å] | 401.844, 401.844, 173.854 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 200.000 * - 3.800 |
R因子 | 0.241 |
Rwork | 0.241 |
R-free | 0.31200 |
Structure solution method | DIFFERENCE FOURIER |
Starting model (for MR) | 1J5E WITHOUT IONS AND PORTIONS AROUND A SITE |
結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
結合角の平均二乗偏差(RMSD) [度] | 1.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 141.420 | 3.940 |
分解能 [Å] (高) | 3.800 * | 3.780 |
Rmerge_l_obs | 0.080 * | 0.295 * |
Total number of observations | 456527 * | |
独立反射数 | 135995 | |
<I/σ(I)> | 7 | 1.7 |
完全性 [%] | 99.3 * | 96.1 * |
冗長性 | 3.4 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | Clemons, W.M., (2001) J. Mol. Biol., 310, 827. * |
結晶化試薬
ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
1 | 1 | 1 | NH4Cl | ||
10 | 1 | 2 | CaCl2 | ||
11 | 1 | 2 | magnesium acetate | ||
12 | 1 | 2 | MPD | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | CaCl2 | ||
4 | 1 | 1 | magnesium acetate | ||
5 | 1 | 1 | sodium cacodylate | ||
6 | 1 | 1 | potassium-MES | ||
7 | 1 | 1 | MPD | ||
8 | 1 | 2 | NH4Cl | ||
9 | 1 | 2 | KCl |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | reservoir | MPD | 14-16 (%(v/v)) | |
2 | 1 | reservoir | 0.2 (M) | ||
3 | 1 | reservoir | 75 (mM) | ||
4 | 1 | reservoir | 15 (mM) | ||
5 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |