1MWD

WILD TYPE DEOXY MYOGLOBIN

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	150
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS
Spacegroup name	I 1 21 1
格子定数 [Å]	122.120, 42.140, 91.490
格子定数 [度]	90.00, 92.85, 90.00

精密化法

残基	24.000 - 1.800
R因子	0.196 *
Rwork	0.196
R-free	0.24300
Structure solution method	OTHER
結合長の平均二乗偏差(RMSD) [Å]	0.013
結合角の平均二乗偏差(RMSD) [度]	0.030
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	CCP4
Refinement software	REFMAC

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	24.000
分解能 [Å] (高)	1.800
Rmerge_l_obs	0.040 *	0.291 *
独立反射数	42068
<I/σ(I)>	21.4
完全性 [%]	96.5
冗長性	7.9

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, hanging drop *	7.1		0.1 PHOSPHATE BUFFER 72-80% SATURATED AMMONIUM SULPHATE, pH 7.1

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	ammonium sulfate	72-80 (%(v/v)sat)
2	1	drop	phosphate	0.1 (M)
3	1	drop	protein	10 (mg/ml)