1MB2

Crystal Structure of Tryptophanyl-tRNA Synthetase Complexed with Tryptophan in an Open Conformation

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
格子定数 [Å]	228.730, 92.240, 157.590
格子定数 [度]	90.00, 132.71, 90.00

精密化法

残基	15.000 * - 2.700
Rwork	0.220
R-free	0.24300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d2r
結合長の平均二乗偏差(RMSD) [Å]	0.009
結合角の平均二乗偏差(RMSD) [度]	1.020 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	15.024	2.800
分解能 [Å] (高)	2.700 *	2.700
Rmerge_l_obs	0.079 *
Total number of observations	120955 *
独立反射数	55705
完全性 [%]	83.1	50.4

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	MICRODIALYSIS	6.6	42 *	potassium phosphate, l-tryptophan, pH 6.6, MICRODIALYSIS, temperature 310K

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	1	protein	4 (mg/ml)
2	1	2	potassium phosphate	2 (M)	pH6.6
3	1	2	Trp	2 (mM)