1M0U
Crystal Structure of the Drosophila Glutathione S-transferase-2 in Complex with Glutathione
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 89.723, 89.723, 131.789 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.000 - 1.750 |
| R-factor | 0.21462 |
| Rwork | 0.213 |
| R-free | 0.23200 * |
| Structure solution method | MIRAS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.190 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.700 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.039 * | 0.238 * |
| Total number of observations | 623131 * | |
| Number of reflections | 62202 | |
| Completeness [%] | 99.5 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 294 | Agianian, B., (2001) Acta Crystallogr., D57, 725. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 20 (mM) | pH7.0 |
| 3 | 1 | drop | beta-mercaptoethanol | 2 (mM) | |
| 4 | 1 | drop | GSH | 15 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.6-1.9 (M) | |
| 6 | 1 | reservoir | sodium phosphate | 0.1 (M) | pH6.0-6.5 |
