1LVM

CATALYTICALLY ACTIVE TOBACCO ETCH VIRUS PROTEASE COMPLEXED WITH PRODUCT

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-01-18
Detector	ADSC QUANTUM 4
Wavelength(s)	0.92
Spacegroup name	P 43 21 2
格子定数 [Å]	75.505, 75.505, 183.167
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	30.000 * - 1.800
Rwork	0.171
R-free	0.22900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lvb
結合長の平均二乗偏差(RMSD) [Å]	0.020
結合角の平均二乗偏差(RMSD) [度]	1.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	30.000 *	1.860
分解能 [Å] (高)	1.800	1.800
Rmerge_l_obs	0.058
独立反射数	49988 *
完全性 [%]	99.8 *	99.3
冗長性	6.2

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	ammonium sulfate, magnesium chloride, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	4.5 (mg/ml)	pH8.5
2	1	reservoir	Tris-HCl	0.1 (M)
3	1	reservoir		0.2 (M)
4	1	reservoir	glycerol	10 (%)
5	1	reservoir	ammonium sulfate	2.0 (M)