1KWB
Crystal structure of the His145Ala mutant of 2,3-dihydroxybipheny dioxygenase (BphC)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-10-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 121.650, 121.650, 109.153 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 84.520 - 2.000 |
R-factor | 0.16642 |
Rwork | 0.166 |
R-free | 0.19100 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.380 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.500 * | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.096 * | 0.254 |
Number of reflections | 27854 | |
<I/σ(I)> | 6.6 | |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 9.7 | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 7.5 | 12 * | Uragami, Y., (2001) J.Inorg.Biochem., 83, 269. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 10 (mg/ml) | |
2 | 1 | 1 | Tris-HCl | 100 (mM) | |
3 | 1 | 1 | ammonium sulfate | 27.7 (%sat) | |
4 | 1 | 1 | hexylene glycol | 18 (%(v/v)) |