1KU2
Crystal Structure of Thermus aquaticus RNA Polymerase Sigma Subunit Fragment Containing Regions 1.2 to 3.1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2000-09-14 |
Detector | BRANDEIS - B4 |
Wavelength(s) | .979 |
Spacegroup name | P 65 |
Unit cell lengths | 104.186, 104.186, 169.181 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.900 |
R-factor | 0.23 |
Rwork | 0.230 |
R-free | 0.27100 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.286 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.037 | 0.147 |
Total number of observations | 97790 * | |
Number of reflections | 23536 | |
<I/σ(I)> | 24.9 | 6.2 |
Completeness [%] | 97.1 | 96.5 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 | 22.5 * | MOPS, ammonium sulfate, magnesium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | MOPS | 0.1 (M) | pH7.0 |
3 | 1 | reservoir | ammonium sulfate | 2 (M) | |
4 | 1 | reservoir | 20 (mM) |