1KP6
USTILAGO MAYDIS KILLER TOXIN KP6 ALPHA-SUBUNIT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 48.300, 48.300, 124.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.164 |
| Rwork | 0.164 |
| R-free | 0.21100 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.309 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.077 | |
| Total number of observations | 109467 * | |
| Number of reflections | 8260 | |
| <I/σ(I)> | 2.15 | |
| Completeness [%] | 93.9 | 90 |
| Redundancy | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | drop1-0.005 ml, drop2-0.001 ml * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop1 | protein | 10 (mg/ml) | |
| 2 | 1 | drop2 | ammonium sulfate | 85 (%) | |
| 3 | 1 | drop2 | MES | 10 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 18-21 (%) | |
| 5 | 1 | reservoir | MES | 10 (mM) |
