1J1V
Crystal structure of DnaA domainIV complexed with DnaAbox DNA
Experimental procedure
| 実験手法 | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9792, 0.9795, 0.9840, 0.9736 |
| Spacegroup name | P 41 21 2 |
| 格子定数 [Å] | 50.385, 50.385, 158.830 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 50.000 - 2.100 |
| R因子 | 0.2292 |
| Rwork | 0.229 |
| R-free | 0.24890 |
| Structure solution method | MAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.150 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.180 |
| 分解能 [Å] (高) | 2.100 | 2.100 |
| Rmerge_l_obs | 0.055 * | 0.110 * |
| 独立反射数 | 12618 | |
| 完全性 [%] | 99.0 * | 98.8 * |
| 冗長性 | 12.5 * |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | Tris-HCl, magnesium formate, polyethylene glycol 8000, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | Tris-HCl | ||
| 2 | 1 | 1 | magnesium formate | ||
| 3 | 1 | 1 | polyethylene glycol 8000 | ||
| 4 | 1 | 2 | Tris-HCl | ||
| 5 | 1 | 2 | magnesium formate | ||
| 6 | 1 | 2 | polyethylene glycol 8000 |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.4 |
| 3 | 1 | reservoir | magnesium formate | 0.2 (M) | |
| 4 | 1 | reservoir | PEG8000 | 18 (%) |
