1ISG
Crystal Structure Analysis of BST-1/CD157 with ATPgammaS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL24XU |
Synchrotron site | SPring-8 |
Beamline | BL24XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-03-18 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 0.836 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.573, 112.851, 130.527 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.600 |
R-factor | 0.211 * |
Rwork | 0.211 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.215 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.068 | 0.371 |
Number of reflections | 26538 | |
<I/σ(I)> | 5.9 | 33.6 |
Completeness [%] | 98.1 | 94.2 |
Redundancy | 31.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 4.5 | 20 * | ammonium sulfate, citrate, ATPgammaS, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | NMN | 62.5 (mM) | |
2 | 1 | 1 | ATP gammaS | 37.5 (mM) | |
3 | 1 | 1 | ethenoNADP | 62.5 (mM) | |
4 | 1 | 1 | ethenoNAD | 62.5 (mM) |