1I31
MU2 ADAPTIN SUBUNIT (AP50) OF AP2 CLATHRIN ADAPTOR, COMPLEXED WITH EGFR INTERNALIZATION PEPTIDE FYRALM AT 2.5 A RESOLUTION
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.006 |
| Spacegroup name | P 64 |
| 格子定数 [Å] | 125.727, 125.727, 74.634 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 20.000 - 2.500 |
| Rwork | 0.247 |
| R-free | 0.25470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bw8 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.019 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.055 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 20.000 | 2.560 |
| 分解能 [Å] (高) | 2.500 | 2.520 |
| Rmerge_l_obs | 0.077 | 0.599 |
| 独立反射数 | 21919 | |
| <I/σ(I)> | 25.8 | 1.6 |
| 完全性 [%] | 96.4 | 76.2 |
| 冗長性 | 50.8 | 6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | SODIUM FORMATE, SODIUM ACETATE, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
