1GRN
CRYSTAL STRUCTURE OF THE CDC42/CDC42GAP/ALF3 COMPLEX.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.400, 69.000, 129.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.25900 |
| Structure solution method | MAD |
| Starting model (for MR) | CDC42 IN THE GTP FORM |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.570 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.200 |
| High resolution limit [Å] | 1.800 | 2.100 |
| Rmerge | 0.064 * | |
| Total number of observations | 129060 * | |
| Number of reflections | 23543 | |
| <I/σ(I)> | 29 | 9.3 |
| Completeness [%] | 85.9 | 85.9 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | pH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 15-20 (%(w/v)) | |
| 2 | 1 | reservoir | magnesium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | 0.10 (mM) | ||
| 4 | 1 | reservoir | 10-30 (mM) |
