1G2X
Sequence induced trimerization of krait PLA2: crystal structure of the trimeric form of krait PLA2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 180 |
Detector technology | IMAGE PLATE |
Collection date | 2000-08-22 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 80.949, 80.572, 57.098 |
Unit cell angles | 90.00, 90.35, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.500 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 1FE5 |
RMSD bond length | 0.007 |
RMSD bond angle | 22.900 * |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 * |
Rmerge | 0.091 * | 0.203 * |
Number of reflections | 12745 | |
<I/σ(I)> | 7.8 | |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 4.98 | 4.98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 * | 281 | 50 mM Tris.HCl, 3.0 M NaCl, 1mM NaN3, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 281.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | pH8.5 |
3 | 1 | drop | 1.4 (M) | ||
4 | 1 | drop | 1 (mM) | ||
5 | 1 | reservoir | 2.8 (M) |