1FQT
CRYSTAL STRUCTURE OF THE RIESKE-TYPE FERREDOXIN ASSOCIATED WITH BIPHENYL DIOXYGENASE
Experimental procedure
| Experimental method | MULTIPLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-04-10 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 0.9537,1.742,1.737 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 76.400, 53.100, 64.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.000 - 1.600 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.20000 |
| Structure solution method | FE MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 27.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.068 | 0.412 |
| Number of reflections | 34971 | |
| <I/σ(I)> | 9.6 | 2.2 |
| Completeness [%] | 98.9 | 97.6 |
| Redundancy | 4.95 | 4.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 10 * | PEG MME 5000, MES buffer, ammonium sulfate, pH 6.3, VAPOR DIFFUSION, SITTING DROP at 283K, pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 31 (mg/ml) | |
| 2 | 1 | drop | sodium chloride | 0.15 (M) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | MOPS | 20 (mM) | |
| 5 | 1 | reservoir | mPEG5000 | 25 (%) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 7 | 1 | reservoir | MES | 0.1 (M) |
