1F5Q
CRYSTAL STRUCTURE OF MURINE GAMMA HERPESVIRUS CYCLIN COMPLEXED TO HUMAN CYCLIN DEPENDENT KINASE 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1998-11-24 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.590, 73.450, 107.670 |
| Unit cell angles | 90.00, 102.18, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.27800 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.410 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.379 |
| Total number of observations | 140317 * | |
| Number of reflections | 45057 * | |
| <I/σ(I)> | 5.7 | |
| Completeness [%] | 97.1 | 95.6 |
| Redundancy | 3.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 298 | 0.1M HEPES, 12% propan-2-ol, 5% PEG 4K, 10mM DTT, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | reservoir | HEPES | 0.1 (M) | |
| 3 | 1 | reservoir | propan-2-ol | 12 (%) | |
| 4 | 1 | reservoir | PEG4000 | 5 (%) | |
| 5 | 1 | reservoir | dithiothreitol | 10 (mM) |
