1EYA
STRUCTURE OF S. NUCLEASE STABILIZING QUINTUPLE MUTANT T33V/T41I/P117G/H124L/S128A
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 297 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 41 |
格子定数 [Å] | 49.181, 49.181, 63.734 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 6.000 - 2.000 |
R因子 | 0.1476 * |
R-free | 0.28850 |
Structure solution method | AB INITIO |
結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
結合角の平均二乗偏差(RMSD) [度] | 0.022 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Quality characteristics
Overall | |
分解能 [Å] (低) | 6.000 * |
分解能 [Å] (高) | 2.000 * |
完全性 [%] | 88.5 * |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | MPD, Sodium phosphate buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | reservoir | MPD | 40-60 (%(v/v)) | |
2 | 1 | reservoir | sodium phosphate | 25 (mM) |