1EYA

STRUCTURE OF S. NUCLEASE STABILIZING QUINTUPLE MUTANT T33V/T41I/P117G/H124L/S128A

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	297
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	P 41
格子定数 [Å]	49.181, 49.181, 63.734
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	6.000 - 2.000
R因子	0.1476 *
R-free	0.28850
Structure solution method	AB INITIO
結合長の平均二乗偏差(RMSD) [Å]	0.006
結合角の平均二乗偏差(RMSD) [度]	0.022
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	SHELXL-97

Quality characteristics

	Overall
分解能 [Å] (低)	6.000 *
分解能 [Å] (高)	2.000 *
完全性 [%]	88.5 *

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	8	277	MPD, Sodium phosphate buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	reservoir	MPD	40-60 (%(v/v))
2	1	reservoir	sodium phosphate	25 (mM)