1E5S
Proline 3-hydroxylase (type II) - Iron form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX7.2 |
Synchrotron site | SRS |
Beamline | PX7.2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-11-15 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 72.780, 72.780, 224.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.820 - 2.400 |
R-factor | 0.198 * |
Rwork | 0.216 |
R-free | 0.22600 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PROLINE HYDROXYLASE APO FORM |
RMSD bond length | 0.047 * |
RMSD bond angle | 3.000 * |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.000 * | 2.360 |
High resolution limit [Å] | 2.400 * | 2.400 * |
Rmerge | 0.101 * | 0.400 * |
Total number of observations | 375824 * | |
Number of reflections | 26230 * | |
<I/σ(I)> | 3 | 1.1 |
Completeness [%] | 93.8 * | 98.4 |
Redundancy | 2.9 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 21 * | 2M AMMONIUM SULFATE, 50MM MES BUFFER, PH6, pH 6.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | MES | 50 (mM) | pH6.0 |
3 | 1 | reservoir | HEPES | 50 (mM) | pH7.5 |
4 | 1 | reservoir | PEG400 | 2 (%) | |
5 | 1 | reservoir | ammonium sulfate | 2.0 (M) |