1E5H

DELTA-R307A DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH SUCCINATE AND CARBON DIOXIDE

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (1.96 Å)

Cell axes106.260106.26071.060
Cell angles90.0090.00120.00
SpacegroupH 3
Resolution limits16.57 - 1.96
the highest resolution shell value2.080 - 1.960
R-factor0.22 (0.220*)
R-work0.22000
the highest resolution shell value0.263
R-free0.25200
the highest resolution shell value0.300
RMSD bond length0.012
RMSD bond angle1.300 (22.700*)

Data Collection Statistics

Resolution limits30.00 - 1.96
the highest resolution shell value -
Number of reflections21766
Number of measurements128961*
Rmerge_l_obs0.090
the highest resolution shell value0.281
Completeness99.8
the highest resolution shell value99.6*
Redundancy5.92

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit
1Vapor diffusion, hanging drop*720**

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoirHEPES-NaOH100 (mM)
21reservoirpotassium 2-oxoglutarate5 (mM)
31reservoirammonium sulfate1.6-1.7 (M)
41dropHEPES-NaOH55 (mM)
51droppotassium 2-oxoglutarate2.5 (mM)
61dropammonium sulfate0.8-0.85 (M)
Annotated Information is extracted from Literature Info*
Lloyd, M.D., (1999) J.Mol.Biol., 287, 943.