1D30
THE STRUCTURE OF DAPI BOUND TO DNA
Experimental procedure
| Detector technology | AREA DETECTOR |
| Detector | SDMS |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 25.350, 40.710, 66.530 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 10.000 - 2.400 |
| R因子 | 0.222 |
| Refinement software | NUCLSQ |
Quality characteristics
| Overall | |
| 分解能 [Å] (高) | 2.400 |
| Rmerge_l_obs | 0.116 * |
| 独立反射数 | 2541 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, sitting drop * | 7 | 4 * | pH 7.00, VAPOR DIFFUSION, temperature 277.00K |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 1 | MG ACETATE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | DNA | 0.28 (mM) | |
| 2 | 1 | drop | spermine-HCl | 0.22 (mM) | |
| 3 | 1 | drop | magnesium acetate | 5.38 (mM) | |
| 4 | 1 | drop | 4'-6-diamidine-2-phenyl indole | 0.23 (mM) | |
| 5 | 1 | drop | MPD | 20 (%(v/v)) | |
| 6 | 1 | reservoir | 40 (%(v/v)) |
